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Single-Cell RNA Sequencing Workflow

Open Single-Cell RNA Sequencing Workflow Fullscreen

About This MicroSim

Chapter 18: Frontier Topics and Emerging Technologies

Library: p5.js

Type: Animated Pipeline Diagram sim-id: scrna-seq-workflow
Library: p5.js
Status: Specified

Animated left-to-right pipeline showing the five steps of scRNA-seq: (1) tissue dissociation into individual cells (show a tissue chunk breaking into separate colored circles), (2) cell capture in droplets (show cells entering small compartments), (3) barcoding (show a unique color-coded tag attaching to RNA molecules inside each droplet), (4) pooled sequencing (show barcoded molecules flowing through a sequencer), (5) computational clustering (show a UMAP-style scatter plot forming with colored clusters). Each step highlights with an animation when the user clicks "Next Step" or plays automatically. Include labels and brief descriptions for each stage.